To trigger an event manually you need to get a reference to the iTweenEvent and then call the Play method on that component. There is a static iTweenEvent method that helps you get your particular event.
iTweenEvent.GetEvent(theGameObjectWithTheiTweenEvent, "name of your event here").Play();
There is an example project here that shows how to do this: http://koontzfamily.org/david/iTween/PlayExample.zip